10x Genomics Multiome ATAC+GEX
Andrew Houston
Published: 2023-05-19 DOI: 10.17504/protocols.io.n2bvj8kqpgk5/v1
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Abstract
Link to 10x Genomics Multiome protocol to assess chromatin accessibility and gene expression from a dissociated sample
Steps
1.
Protocol can be found here:
http://cdn.10xgenomics.com/image/upload/v1666737555/support-documents/CG000338_ChromiumNextGEM_Multiome_ATAC_GEX_User_Guide_RevF.pdf
Transposition through cDNA pgs. 28-57
ATAC library construction pgs. 49-52
GEX library construction pgs. 59-67
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