10x Protocols: Visium v1 FFPE Deparaffinization, H&E staining, and Imaging -- University of Minnesota TMCs (CG000409 Rev B)
IOx Genomics, Laura J Niedernhofer, David A Bernlohr
Abstract
Protocols from 10x Genomics for Visium Spatial Gene Expression v1 chemistry on FPPE samples without the CytAssist component.
Protocol ID# (CG) and Revision letter provided here :
10x protocol CG000409, **Revision B** -- Deparaffinization, H&E staining, coverslipping, and imaging
Additional protocols added for guidance:
10x protocol for imaging guidelines
10x protocol for tissue preparation guidelines
Note: These protocols may not be the current version offered by 10x Genomics but were used to produce the specific datasets connected to them. Please review the 10x Genomics support website for the most recent versions of the protocols prior to starting your experiment.
Steps
10x protocol CG000409, Rev B (Deparaffinization, H&E, etc)
CG000409_Demonstrated_Protocol_VisiumSpatialFFPE_Deparaffin_H&E_RevB.pdf
Additional Protocols/Resources
10x Manual Visium FFPE Imaging Guidelines, Rev A (Protocol CG000436).pdf
10x Manual Visium Spatial FFPE Tissue Preparation Guide.pdf
http://www.10xgenomics.com/support/spatial-gene-expression-ffpe
