ABT263 (Navitoclax) Treatment in Mice
Andrew Davis, Peter Adams
Published: 2023-05-14 DOI: 10.17504/protocols.io.5jyl8j4r6g2w/v1
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Abstract
This protocol is for treating mice with the senolytic, Navitoclax, by oral gavage.
Before start
ABT263 must first be dissolved in the vehicle by heating to 60°C. Sonication can be used to dissolve faster. See the materials section for specifics.
Steps
1.
Give mice Navitoclax or Vehicle by oral gavage at 50mg/kg/day. Mice receive vehicle or drug for a total of 14 days, spread across 6 weeks, as outlined in the sub-steps below.
1.1.
Mice receive seven days of once-daily oral gavage of drug or vehicle
1.2.
Then fourteen days with no treatment
1.3.
Then seven more days of once-daily oral gavage of drug or vehicle
1.4.
Then fourteen days with no treatment
1.5.
Harvest tissues after the last day of no treatment
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