Adolf & Du et al., 2024
Frank Adolf
Published: 2024-02-07 DOI: 10.17504/protocols.io.e6nvwdrozlmk/v1
ASAPCRN
proteasome
core particle
20S proteasome
chaperone
molecular machine
multiprotein complex
POMP
PAC1
PAC2
PAC3
PAC4
propeptide
protease
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Abstract
These protocols details methods for cloning, expression, purification and structural determination by transmission electron cryo-microscopy of 20S CPs and assembly intermediates.
Steps
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