The recipe produces enough medium to fill approximately 18 culture tubes.(12cm)

First, add powder or solid

A	B
component	amount
streptomycin	0.175 g
Wesson's salt mixture	0.625 g
yeast	4 g
corn starch	4 g
casein	5 g
agar	15 g
sucrose	2.5 g
redbay sawdust	100 g

Second, add liquid

A	B
component	amount
wheat germ oil	1.25ml
95％ ethanol	2.5ml
deionized water	290ml

All ingredients were mixed thoroughly, then put cotton plugs into the mouth of the filled tubes.

The culture tubes was autoclaved at 121 °C for 30 min.

Put culture tubes to dry 2 days in oven at 55°C.

(cotton plugs don't remove)