The recipe is for filling c.a. 18⁠—20 falcon tubes (⁠50ml).

First, add dry ingredients

A	B
component	amount
cycloheximide	0.175 g
Wesson's salt mixture	0.625 g
yeast	4 g
corn starch	4 g
casein	5 g
agar	15 g
sucrose	2.5 g
redbay sawdust	100 g

Second, add wet ingredients

A	B
component	amount
wheat germ oil	1.25ml
95％ ethanol	2.5ml
deionized water	290ml

Mix all the ingredients thoroughly and fill in 50ml falcon tubes.

To compress the media, use a clean 15ml falcon tube with a cap on, and press the media until it gets compressed.

Put a double-layered cotton gauze on the mouth of the filled tubes. The cap is pre-drilled with holes that allow aeration and prevent contamination.

Autoclave the filled tube at 121 °C for 30 min.

Put autoclved tubes in an oven at 55°C for two days. (cotton gauze and cap remains on).

Happy beetle rearing!