Add 100µL of magnetic beads (10 mg/ml) to the 1st well of 96 deep well plate

Add 200µL of 0.5 mm beads to 2mL screw tube

Add200µL of 1 mm beads to 2mL screw tube

Add870µL Lysis master mix to 2mL screw tube. The final look:

Collect 20-50mg of sample to 2mL screw tube

Put the 2mL screw tube in mixmill for sample crush, at 3200 rpm 0h 4m 0s

Put 2mL screw tube in centrifuge for centrifugation, at this condition:10x g,25°C

Add 350µL of isopropanol to the 1st well of 96 well plate

Add 50µL of magnetic beads (10mg/ml) to the 1st well of 96 deep well plate

Add 400µL of isopropanol to the 2nd well of 96 deep well plate

Add 300µL of 80% ethanol to the 3rd well of 96 deep well plate

Add 300µL of 95% ethanol to the 4th well of 96 deep well plate

Add 300µL of DDW to the 5th well of 96 deep well plate

Add 100µL of DEPC-treated water to the 6th well of 96 deep well plate

Add 300-500µL of the sample (lysate) from the 1.5mL centrifuged tube to the 1st well of 96 deep well plate

Put the prepared 96 deep well plate in the automated DNA extraction machine and select the BOMB protocol

After the extraction is done, put on the 96 magnetic plate to pellet the magnetic bead residues.

Collect 100µL of the eluted sample (avoid getting magnetic bead) as the DNA template for downstream experiments