Desynchronizing the sleep–wake cycle from circadian timing to assess their separate contributions to physiology and behaviour and to estimate intrinsic circadian period

Wei Wang, Robin K. Yuan, Jude F. Mitchell, Kirsi-Marja Zitting, Melissa A. St. Hilaire, James K. Wyatt, Frank A. J. L. Scheer, Kenneth P. Wright, Emery N. Brown, Joseph M. Ronda, Elizabeth B. Klerman, Jeanne F. Duffy, Derk-Jan Dijk, Charles A. Czeisler

Published: 2022-11-13 DOI: 10.1038/s41596-022-00746-y

Circadian Rhythms

Forced Desynchrony

Intrinsic Period

Sleep-Wake Cycle

AI 解读

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Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Circadian clocks drive cyclic variations in many aspects of physiology, but some daily variations are evoked by periodic changes in the environment or sleep–nobreakwake state and associated behaviors, such as changes in posture, light levels, fast
Introduction
Materials
Procedure 1: the standard procedure
Procedure 2: the real-life case study procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Desynchronizing the sleep­­–wake cycle from circadian timing to assess their separate contributions to physiology and behaviour and to estimate intrinsic circadian period

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Desynchronizing the sleep–wake cycle from circadian timing to assess their separate contributions to physiology and behaviour and to estimate intrinsic circadian period