Differentiation of SH-SY5Y cells
Shenjie Wu, Nancy C. Hernandez Villegas, schekman
Published: 2023-08-26 DOI: 10.17504/protocols.io.dm6gp3jj1vzp/v1
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Abstract
This protocol describes a standard procedure to differentiate SH-SY5Y cells into dopaminergic neurons using retinoic acid
Steps
Differentiation of SH-SY5Y cells
1.
SH-SY5Y neuroblastoma cells were maintained in DMEM supplemented with 1× nonessential amino
acid (NEAA), 1× sodium pyruvate, and 10% FBS.
2.
Differentiation was induced by lowering the FBS in culture medium to 1% plus 10 μM RA.
3.
Cell medium was replaced each 3 days to replenish RA.
4.
Cell morphology was monitored by microscopy and experiments on SH-SY5Y cells were performed from D6 of differentiation.
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