Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

Zhiming Li, Xu Hua, Albert Serra-Cardona, Xiaowei Xu, Zhiguo Zhang

Published: 2021-04-27 DOI: 10.1038/s41596-021-00520-6

mammalian cells

chromatin replication

strand-specific profiling

nascent DNA

enrichment sequencing

AI 解读

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Data integration（数据集成）

Heterogeneity（异质性）

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Faithful duplication of both genetic and epigenetic information is essential for all eukaryotic cells. DNA replication initiates from replication origins and proceeds bidirectionally but asymmetrically, with the leading strand being synthesized continuous
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

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