Fluorescence size exclusion chromatography (FSEC) of native nanodiscs
Caroline Brown, Snehasish Ghosh, Kallol Gupta
Published: 2024-05-10 DOI: 10.17504/protocols.io.14egn676ml5d/v1
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Abstract
This is a protocol describing how to conduct Fluorescence size exclusion chromatography of native nanodiscs.
Steps
1.
Conduct extraction into native nanodiscs following established protocols.
2.
Inject 200uL native nanodiscs onto a Superose6 (10/300GL) column which is pre-equilibrated with TBS.
3.
Perform separation at a flow rate of 0.5 mL/min and detect eluent with a Shimadzu fluorometer with excitation set to 488 nm and emission set to 509 nm.
4.
Collect eluent for downstream analysis.
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