Global mapping of RNA G-quadruplexes (G4-RNAs) using G4RP-seq

Sunny Y. Yang, David Monchaud, Judy M. Y. Wong

Published: 2022-02-08 DOI: 10.1038/s41596-021-00671-6

G-quadruplexes

G4RP-seq

RNA profiling

cross-linking

BioTASQ

AI 解读

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Heterogeneity（异质性）

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Guanine-rich RNAs can fold into four-stranded structures, termed G-nobreakquadruplexes (G4-RNAs), and participate in a wide range of biological processes. Here we describe in detail a G4-RNA-specific precipitation (G4RP) protocol, which enables the transc
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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Global mapping of RNA G-quadruplexes (G4-RNAs) using G4RP-seq

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