Glucose Tolerance Test
Sabina Marciano, Roberta Marongiu
Published: 2023-10-13 DOI: 10.17504/protocols.io.261geddodv47/v1
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Abstract
The glucose tolerance test is performed to determine how quickly the glucose is cleared from the blood. It is used to test for diabetes or insulin resistance.
Steps
1.
Single cage the mice for 1 week in advance
2.
Weight the mice
3.
Fast mice for 6h 0m 0s
4.
After fasting, measure glucose with a glucose meter
5.
Perform an intraperitoneal injection of 2g / kg body weight of glucose (20% D-glucose stock solution dissolving 2g of glucose in 10ml saline and give 10ul per gram of body weight).
6.
Collect blood samples for glucose measurement at 15, 30, 45, 60, 90 and 120 min post glucose injection
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