This protocol is designed for large scale laboratory rearing of house flies at very low cost.

If you are collecting flies from a field site to begin your house fly colony, be sure to keep field-collected adult flies away from any existing fly colonies or colony supplies. Field flies are likely to carry several fungal and bacterial pathogens that can infect your existing fly colonies. Instead, hold field flies separately and collect eggs from these flies that can then be placed into your colony rearing rooms without infecting other colonies.

House Fly (Musca domestica) lab rearing protocol.pdf