Intestinal organoid cocultures with microbes

Hans Clevers, Jens Puschhof, Joep Beumer, Cayetano Pleguezuelos-Manzano, Adriana Martinez-Silgado, Ninouk Akkerman, Aurelia Saftien, Charelle Boot, Amy de Waal, Devanjali Dutta, Inha Heo

Published: 2021-08-10 DOI: 10.1038/s41596-021-00589-z

fluorescence microscopy

organoid coculture

host-microbe interaction

microinjection

RNA sequencing

AI 解读

Generation of mouse–human chimeric embryos

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Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

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Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Adult-stem-cell-derived organoids model human epithelial tissues exvivo, which enables the study of host–nobreakmicrobe interactions with great experimental control. This protocol comprises methods to coculture organoids with microbes, particular
Introduction
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- Reporting Summary
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References
ACKNOWLEDGMENTS

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Intestinal organoid cocultures with microbes

Supplementary information

Reporting Summary

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