Microfluidics materials are of various types and application-specific. PDMS is one of the most preferred and cost-effective solutions for research and low-volume manufacturing. After having the mold, PDMS replicas are generated by a technique called soft-lithography. This protocol describes the preparation of PDMS microchannels using SU8 molds or 3D Printed molds by the soft lithography technique.

This protocol is also called "soft lithography" in the literature the reference protocol is taken from the manual of Sylgard 184 silicone elastomer, Dow-Corning.