Off-the-shelf proximity biotinylation using ProtA-TurboID

Irene Santos-Barriopedro, Guido van Mierlo, Michiel Vermeulen

Published: 2022-10-11 DOI: 10.1038/s41596-022-00748-w

Proteomics

Mass spectrometry

Proximity biotinylation

TurboID

Protein A

AI 解读

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Heterogeneity（异质性）

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Proximity biotinylation is a commonly used method to identify the invivo proximal proteome for proteins of interest. This technology typically relies on fusing a bait protein to a biotin ligase using overexpression or clustered regularly interspaced shor
Introduction
Materials
Procedure
Troubleshooting
Anticipated results
Timing
- Reporting summary
- References
References
ACKNOWLEDGMENTS

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Off-the-shelf proximity biotinylation using ProtA-TurboID

Supplementary information

Reporting Summary

Supplementary Table 1

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