Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs)

Shuai Jin, Qiang Gao, Caixia Gao, Qiupeng Lin

Published: 2022-11-24 DOI: 10.1038/s41596-022-00773-9

Prime Editing

PlantPegDesigner

Engineered PEs

Monocot Plants

Optimized pegRNAs

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

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Decoding personal biotic and abiotic airborne exposome

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Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Prime editors (PEs), which can install desired base edits without donor DNA or double-strand breaks, have been used in plants and can, in principle, accelerate crop improvement and breeding. However, their editing efficiency in plants is generally low. Op
Introduction
Materials
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Anticipated results
References
ACKNOWLEDGMENTS

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Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs)

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