Precise genome engineering inPseudomonasusing phage-encoded homologous recombination and the Cascade–Cas3 system

Xue Wang, Wentao Zheng, Yandong Xia, Shiqing Gao, Diao Zhou, Vinothkannan Ravichandran, Chanjuan Jiang, Qiang Tu, Yulong Yin, Youming Zhang, Jun Fu, Ruijuan Li, Jia Yin

Published: 2023-08-24 DOI: 10.1038/s41596-023-00856-1

Pseudomonas genome engineering

Phage-encoded homologous recombination

Cascade-Cas3 system

Recombineering efficiency

Genetic manipulation

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Sections

Figures

References

Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Determination of the resistance and transformation characteristics of Pseudomonas
- Two-step seamless modification and three-step seamless modification
Acknowledgements

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Precise genome engineering inPseudomonasusing phage-encoded homologous recombination and the Cascade–Cas3 system

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