Preparation of frozen nuclei for single-nucleus RNA seq
Kimberly Siletti, Sten Linnarsson
Published: 2022-10-11 DOI: 10.17504/protocols.io.eq2lyrdpgx9k/v2
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Abstract
This protocol describes our preparation of nuclei for single-nucleus RNA sequencing on the 10X Genomics Chromium system, starting from FAC-sorted frozen nuclei.
Before start
Attachments
Single Nuclei Prep for 10X 2019 v2.pdf
Steps
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