Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery

Xu Wang, Shuai Liu, Yehui Sun, Xueliang Yu, Sang M. Lee, Qiang Cheng, Tuo Wei, Junyu Gong, Joshua Robinson, Di Zhang, Xizhen Lian, Pratima Basak, Daniel J. Siegwart

Published: 2022-10-30 DOI: 10.1038/s41596-022-00755-x

Selective Organ Targeting

Lipid Nanoparticles

mRNA Delivery

Nucleic Acid Therapeutics

Scalable Synthesis

AI 解读

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Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

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Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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A new methodology termed selective organ targeting (SORT) was recently developed that enables controllable delivery of nucleic acids to target tissues. SORT lipid nanoparticles (LNPs) involve the inclusion of SORT molecules that accurately tune delivery t
Introduction
Materials
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ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery

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