Protocols for clinical use atNature Protocols Published: 2021-05-10 DOI: 10.1038/s41596-021-00557-7 AI 解读 浏览 1311 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Generation of mouse–human chimeric embryos Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues Decoding personal biotic and abiotic airborne exposome A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst Neuroscience robotics for controlled induction and real-time assessment of hallucinations Scanorama: integrating large and diverse single-cell transcriptomic datasets Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序） Data integration（数据集成） Heterogeneity（异质性） 查看全部 Intrahepatic implantation of tumor cells Intrahepatic tumor implantation Differential transcript usage analysis Small volume fungal genomic DNA extraction protocol for Illumina genome BIDMC TMC - low input single nuclei sequencing isolation (NNLB) Preparation of 1M calcium chloride solution (CaCl2) ngm worm c.elegans c elegans elegans caenorhabditis media medium calcium chloride Gradient PCR with DMSO gDNA RNA Clean Up Protocol Processamento de actígrafos pré-coleta - ActTrust actigrafia sono cronobiologia condor instruments Multiplex PCR Assay for Clade-typing Salmonella Enteritidis Non-typhoidal Salmonella Real-time PCR Phylogeny Molecular surveillance Generating a low-copy overexpression cell line using PhiC31 integration phiC31 overexpression attb 查看全部 ezTrack—A Step-by-Step Guide to Behavior Tracking behavioral analysis fear conditioning freeze position tracking PROMIS: Global Analysis of PROtein-Metabolite Interactions complexes metabolomics protein–metabolite interactions proteomics size-exclusion chromatography Rearing and Experimental Uses of Daphnia: Controlling Animal Nutrition and Assessing Lifespan and Life-History Traits aging culturing Daphnia longevity model organisms nutrition Solid-Supported Membrane (SSM)-Based Electrophysiology Assays Using Surface Electrogenic Event Reader Technology (SURFE²R) in Early Drug Discovery drug discovery high-throughput screening solid-supported membrane (SSM)-based electrophysiology SURFE²R assay Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells ENS progenitors human pluripotent stem cells hPSCs vagal neural crest Establishing a Microfluidic Tumor Slice Culture Platform to Study Drug Response breast cancer chemotherapy microfluidics organ-on-chip predictive assay Single Nucleotide Polymorphism (SNP) Arrays and Their Sensitivity for Detection of Genetic Changes in Human Pluripotent Stem Cell Cultures copy-number variant (CNV) genetic changes human pluripotent stem cells (hPSCs) sensitivity SNP arrays Analysis of Tomato Post-Harvest Properties: Fruit Color, Shelf Life, and Fungal Susceptibility Botrytis fruit color fruit quality fruit storage fungal susceptibility post-harvest analysis shelf life UniLectin, A One-Stop-Shop to Explore and Study Carbohydrate-Binding Proteins carbohydrate glycan-binding lectin structural biology A New Method of Bone Stromal Cell Characterization by Flow Cytometry bone marrow cell sorting flow cytometry osteoblast osteoclast osteocyte 查看全部
