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Scalable characterization of the PAM requirements of CRISPR–Cas enzymes using HT-PAMDA

Russell T. Walton, Jonathan Y. Hsu, J. Keith Joung, Benjamin P. Kleinstiver

Published: 2021-02-04 DOI: 10.1038/s41596-020-00465-2

genome editing

CRISPR-Cas enzymes

HT-PAMDA

PAM preferences

scalable characterization

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The continued expansion of the genome-editing toolbox necessitates methods to characterize important properties of CRISPR–nobreakCas enzymes. One such property is the requirement for Cas proteins to recognize a protospacer-adjacent motif (PAM) in
Introduction
Materials
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Troubleshooting
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Anticipated results
References
ACKNOWLEDGMENTS

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关键词

genome editing

CRISPR-Cas enzymes

HT-PAMDA

PAM preferences

scalable characterization

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