Log into Bankit with UCSF username and password:

- Follow this link: http://www.ncbi.nlm.nih.gov/WebSub/?form=history&tool=genbank

- Choose more login options and select University of California, San Francisco

Select "Start Bankit Submission"

Fill out the contact information

Sequence Authors:

List all sequence authors (use the Add button for more than one)

Reference Information #1:

Select publication status (unpublished, in-press, or Published)

- If in-press add reference title, journal title, year, volume, issue, and page numbers

- If published add reference title, journal title, year, volume, issue, page numbers, and PubMed ID

- Optional: if unpublished add reference title

Select reference authors as "Same as Sequence Authors" or "Specify New Authors"

- If specify new authors, list authors

(Optional) Add Another Reference:

Follow Reference Information #1 steps

Sequencing Technology:

Select the sequencing technology (or other and specify)

Select unassembled sequence reads or assembled sequences (consisting of two or more sequence reads)

List assembly program and version or date

Example: Illumina, Geneious v. Geneious Prime 2023.0.1

Submission Release Date:

Select "Immediately after processing" or "Release date"

- If Release Date is selected, specify date in DD-Mon-YYYY format

Sequence(s) and Definition Line(s):

Choose molecule type, linear or circular, if it is a complete sequence, and fasta or alignment format

Either download sequence file or copy and paste it in the box

- Note: make sure the first line follows this format; make everything in bold specific to your submission

        >Seq **\#** [organism= **genus species** ]  **species straininfo** 

Example: >Seq1[organism=Enterovirus rhinovirus] Rhinovirus C17 strain RvC17/USA/2019

Submission Category:

Select "Original" or "Third Party Annotation"

Source Modifiers:

Choose organelle/ if applicable

Choose source modifiers and list information in value column, add as many as desired

Example: Strain = RvC17/USA/2019, host = homo sapiens, tissue_type = Lung, country = USA, collection_date = 2019

Features (Overview):

Select "Add features by completing input forms"

Select "Coding Region (CDS) / Gene / mRNA"

Select "providing intervals"

Click Add

Features (Detail):

Choose if the CDS is on the + or - strand

If the CDS is not compete, select 5' or 3' for which end it is incomplete at

Select "Entire Sequence" or "Specific Spans" for Nucleotide Interval Spans

- If Specific Spans, specify start and stop and which strand it is on

List protein name (optional: also list protein description and EC number)

Click accept

Example: specific spans: start = 19 stop = 6498 strand = -minus; protein name = polyprotein

Review Submission:

Make sure everything looks good

If resubmitting check the box under the resubmission section