SureSelect XT HS2 DNA to prepare libraries for single-cell Whole Genome Sequencing (scWGS) after single-cell Whole Genome Amplification (scWGA)

Disclaimer

Acknowledgements and Funders:

We thank ICH.ZCR UCL Genomics Sequencing and Agilent for technical support.

This research was funded in part by Aligning Science Across Parkinson’s [Grant ID: 000430] through the Michael J. Fox Foundation for Parkinson’s Research (MJFF).

Note

ALL IMAGES CAN BE ENLARGED BY CLICKING AT THEM Critical notes! Please follow Good Laboratory Practices. To prevent DNA contamination, clean all surfaces and equipment before use with DNA AWAY Surface Decontaminant, separate the lab in pre- and post-PCR areas, and use filtered sterile pipette tips.For each protocol step that requires theremoval of tube cap strips, reseal the tubes with a fresh strip of caps.In all master mixes to be used manually, include 5-10% excess volume in the calculations.

Abstract

We adapted the SureSelect XT HS2 DNA protocol to prepare libraries using as input material samples after single-cell Whole Genome Amplification (scWGA), instead of genomic DNA samples, for library preparation for Illumina single-cell Whole Genome Sequencing (scWGS). This protocol can be employed either manually (Section 2: Option A) or in combination with automation using the Bravo Automated Liquid Handling Platform (Section 2: Option B).

Attachments

861-2221.pdf 2022_SureSelect_Automation_G9985-90020.pdf SureSelect Enzymatic Fragmentation kit.pdf SureSelect XT HS2 DNA.pdf

Disclaimer

Abstract

Attachments

Steps

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