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    TS Spurr's - primary fixation Karnovsky's - tissue (TM - 013)

    sandra.crameri

    Published: 2023-05-09 DOI: 10.17504/protocols.io.8epv5j45jl1b/v2
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    Abstract

    This method is used for conventional processing of tissue to Spurr's resin.

    Steps

    1.

    SAN:

    SPEC No:

    OPERATOR & STEPS:

    OPERATOR & STEPS:

    CONVENTIONAL

    2.

    2.5% volume plus 4% volume in 0.1Molarity (M) for at least 0h 40m 0s

    3.

    Wash 0.1Molarity (M) Sorenson's Phosphate Buffer 07.2 (300mosmol/kg) for 0h 15m 0s

    4.

    1% volume in buffer for 1h 0m 0s

    5.

    70% volume for at least 0h 20m 0s

    6.

    95% volume for 0h 20m 0s

    7.

    100% volume for 0h 20m 0s

    8.

    100% volume dried molecular sieved for 0h 20m 0s

    9.

    50% volume 50% volume for 0h 30m 0s

    10.

    100% volume 0h 30m 0s

    11.

    100% volume 1h 0m 0s

    12.

    Oven polymerise overnight at 65°C

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