• 云顶集团3118(中国)

    Tissue Culture (tc) PBS

    Jacquelina.Woods

    Published: 2021-12-02 DOI: 10.17504/protocols.io.byajpscn
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    Abstract

    This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater​​. Protocols developed for this project cover wastewater collection, concentration, RNA extraction, RT-qPCR detection, library prep, genome sequencing, quality control checks, and data submission to NCBI. This method provides a reagent formula required in the rapid concentration of intact viruses from wastewater using a combination of PEG precipitation and ultracentrifugation.

    Steps

    1X PBS

    1.

    Dissolve components in deionized or ultrapure water to 1L.

    1.1.

    8.0g

    1.2.

    0.2g

    1.3.

    0.12g

    1.4.

    0.91g

    2.

    Adjust 7.5

    3.

    Autoclave 121°C``0h 15m 0s

    4.

    Store at 2-8°C

    Note
    For 2X tc PBS, double components in 1.1 - 1.4

    10X tc PBS

    5.

    Note
    Alternatively, 1X and 2X PBS can be made by dilution of 10X PBS into sterile deionized or ultrapure water.

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