Transgenic mouse colony maintenance
Ariadna Laguna, Miquel Vila, Iria Carballo-Carbajal
Published: 2024-06-18 DOI: 10.17504/protocols.io.yxmvmeyx5g3p/v1
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Abstract
Methods for the generation and maintenance of transgenic tyrosinase mice
Steps
1.
Pronuclear microinjection of the human
tyrosinase complementary DNA fused to the rat tyrosine hydroxylase promoter into C57Bl6-SJL (RRID:MGI:5656718) mouse zygotes
2.
Selection of positive lines and backcrossing for 8-10 generations using
C57BL/6J mice (Charles River; RRID:MGI:3028467)
3.
Maintainance in heterozygosis by crossing heterozygous males with C57BL76J females
4.
Housed two to five per cage with ad libitum
access to food and water during a 12 hours (h) light/dark cycle (light-on 8
a.m.)
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