Tween 60-esculin Agar (TE slant)
Amy Gladfelter
Published: 2023-06-02 DOI: 10.17504/protocols.io.yxmvm73e5v3p/v1
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Abstract
This media is used to determine ability of Malassezia species to hydrolyzeesculin and utilize Tween 60.
Before start
Attachments
Malassezia media_1.pdf
Steps
1.
peptone 10g
2.
glucose 10g
3.
yeast extract 2g
4.
ferric ammonium citrate 0.5g
5.
esculin 1g
6.
agar 15g
7.
Tween 60 5mL
8.
dH2O 1L
9.
Sterilize by autoclaving.
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