Utilizing Skyline to analyze lipidomics data containing liquid chromatography, ion mobility spectrometry and mass spectrometry dimensions

Kaylie I. Kirkwood, Brian S. Pratt, Nicholas Shulman, Kaipo Tamura, Michael J. MacCoss, Brendan X. MacLean, Erin S. Baker

Published: 2022-07-12 DOI: 10.1038/s41596-022-00714-6

Lipidomics

Skyline

LC-IMS-CID-MS

Data annotation

Open-source software

AI 解读

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Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

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Neuroscience robotics for controlled induction and real-time assessment of hallucinations

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Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Lipidomics studies suffer from analytical and annotation challenges because of the great structural similarity of many of the lipid species. To improve lipid characterization and annotation capabilities beyond those afforded by traditional mass spectromet
Introduction
- Comparison with other methods
- Limitations
Materials
- Equipment
Procedure
Troubleshooting
Timing
Anticipated results
- References
- References
References
ACKNOWLEDGMENTS

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Kirkwood, K. I. et al. Utilizing Skyline to analyze lipidomics data containing liquid chromatography, ion mobility spectrometry and mass spectrometry dimensions. Zenodo http://zenodo.org/record/6374209#.YpzYhBPMJJU (2022).

Utilizing Skyline to analyze lipidomics data containing liquid chromatography, ion mobility spectrometry and mass spectrometry dimensions

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