Tutorial: using nanoneedles for intracellular delivery

Ciro Chiappini, Yaping Chen, Stella Aslanoglou, Anna Mariano, Valentina Mollo, Huanwen Mu, Enrica De Rosa, Gen He, Ennio Tasciotti, Xi Xie, Francesca Santoro, Wenting Zhao, Nicolas H. Voelcker, Roey Elnathan

Published: 2021-08-22 DOI: 10.1038/s41596-021-00600-7

nanoneedles

intracellular delivery

therapeutic nucleic acids

nanoinjection workflow

bioassays

AI 解读

Supplementary information

Supplementary Information

Supplementary Table 1

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Figures

References

Supplementary information

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Tutorial: using nanoneedles for intracellular delivery

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